Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photo-affinity label for cellular retinol-binding protein.

M Sheves, A Makover, S Edelstein

    Biochemical and Biophysical Research Communications
    |July 31, 1984
    PubMed
    Summary

    Researchers developed 4-azidoretinol, a photo-reactive derivative, to label cellular retinol-binding protein. This compound covalently binds to the protein upon irradiation, aiding in studying retinoid interactions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    BNT 13b2 Pfizer vaccine protects against SARS-CoV-2 respiratory mucosal colonization even after prolonged exposure to positive family members.

    The Journal of hospital infection·2021
    Same author

    Measurements of the stabilities of isolated retinal chromophores.

    Physical review. E·2017
    Same author

    Risk factors for persistent disability in children with obstetric brachial plexus palsy.

    Journal of perinatology : official journal of the California Perinatal Association·2016
    Same author

    Effects of Continuous Octreotide Infusion on Intraoperative Transfusion Requirements During Orthotopic Liver Transplantation.

    Transplantation proceedings·2015
    Same author

    Erratum: Synthetic retinal analogs modify the spectral and kinetic characteristics of microbial rhodopsin optogenetic tools.

    Nature communications·2015
    Same author

    Synthetic retinal analogues modify the spectral and kinetic characteristics of microbial rhodopsin optogenetic tools.

    Nature communications·2014

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Organic Chemistry

    Background:

    • Cellular retinol-binding protein (CRBP) plays a crucial role in retinoid metabolism and transport.
    • Developing specific labeling agents is essential for studying protein-ligand interactions.

    Purpose of the Study:

    • To synthesize and characterize novel retinoid derivatives for labeling CRBP.
    • To investigate the binding affinity and photo-reactive properties of these derivatives.

    Main Methods:

    • Synthesis of various retinoid derivatives.
    • Competition binding assays with all-trans-retinol and CRBP.
    • Photo-crosslinking studies using UV irradiation.
    • SDS-gel electrophoresis to assess covalent attachment.

    Main Results:

    • Most retinoid derivatives failed to bind CRBP.
    • 4-azidoretinol demonstrated high relative binding affinity for CRBP, competing effectively with all-trans-retinol.
    • Irradiation of the 4-azidoretinol-CRBP complex resulted in stable, covalent attachment, confirmed by SDS-gel electrophoresis.

    Conclusions:

    • 4-azidoretinol is a potent photo-reactive label for CRBP.
    • This derivative enables covalent labeling of CRBP, facilitating further structural and functional studies.
    • A method for synthesizing 4-azidoretinol was successfully established.

    Related Experiment Videos