Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Computer-assisted grain counting for autoradiography.

E Y Kam, J D Pitts

    Computer Programs in Biomedicine
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Metabolic co-operation between biochemically marked mammalian cells in tissue culture. 1969.

    Reviews in medical virology·2002
    Same author

    In vivo NADH fluorescence monitoring as an assay for cellular damage in photodynamic therapy.

    Photochemistry and photobiology·2002
    Same author

    Autofluorescence characteristics of immortalized and carcinogen-transformed human bronchial epithelial cells.

    Journal of biomedical optics·2001
    Same author

    Binding of bovine papillomavirus type 4 E8 to ductin (16K proteolipid), down-regulation of gap junction intercellular communication and full cell transformation are independent events.

    The Journal of general virology·2000
    Same author

    Interactions between normal mammary epithelial cells and mammary tumour cells in a model system.

    Cell proliferation·2000
    Same author

    Structure of the ductin channel.

    Bioscience reports·1999
    Same journal

    Microprocessor-based system for on-line analysis of respiratory responses to exercise.

    Computer programs in biomedicine·1985
    Same journal

    A calculator program for clinical application of the Bayesian method of predicting plasma drug levels.

    Computer programs in biomedicine·1985
    Same journal

    Testing marginal homogeneity in square tables; with emphasis on matched data.

    Computer programs in biomedicine·1985
    Same journal

    Computerized analysis of enzyme cascade reactions using continuous rate data obtained with an ELISA reader.

    Computer programs in biomedicine·1985
    Same journal

    Mathematical modelling of stimulus-secretion coupling in the pancreatic B-cell. II. Calcium-stimulated calcium release.

    Computer programs in biomedicine·1985
    Same journal

    A program for logistic prediction modelling.

    Computer programs in biomedicine·1985
    See all related articles

    This study introduces low-cost, accurate, and reproducible software for automated autoradiography grain counting using the System III Image Analyser. The programs can be adapted for various particle counting applications.

    Area of Science:

    • * Scientific Imaging and Analysis
    • * Microscopy and Image Processing
    • * Nuclear Medicine and Biology

    Background:

    • * Autoradiography is a crucial technique for visualizing and quantifying the distribution of radioactive substances in biological samples.
    • * Manual counting of autoradiographic grains is labor-intensive, prone to errors, and lacks reproducibility.
    • * Existing automated systems can be expensive and may not be flexible for diverse applications.

    Purpose of the Study:

    • * To develop and present two cost-effective software programs for the System III Image Analyser.
    • * To partially automate the grain counting process in autoradiography.
    • * To present quantitative data in histogram form for improved data analysis.

    Main Methods:

    • * Utilization of the System III Image Analyser for image acquisition.

    Related Experiment Videos

  • * Development of custom software algorithms for automated particle (grain) detection and counting.
  • * Implementation of data visualization tools to generate histograms of particle distribution.

    • Main Results:

    • * Successful partial automation of the autoradiography counting process.
    • * Achieved high accuracy and reproducibility in grain counting.
    • * Demonstrated the system's cost-effectiveness compared to existing solutions.

    Conclusions:

    • * The developed programs offer an affordable and reliable solution for autoradiography analysis.
    • * The software's adaptability allows for its application in other particle counting tasks.
    • * This automated approach enhances the efficiency and precision of quantitative imaging studies.