Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural and dynamic differences between supercoiled and linear DNA from proton NMR.

P Bendel, T L James

    Proceedings of the National Academy of Sciences of the United States of America
    |June 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Three-dimensional RNA structure-based drug discovery.

    Journal of biomolecular structure & dynamics·2012
    Same author

    Atrophic enlargement of CSF volume after subarachnoid hemorrhage: correlation with neuropsychological outcome.

    AJNR. American journal of neuroradiology·2009
    Same author

    Motor cortex and thalamic atrophy in Unverricht-Lundborg disease: voxel-based morphometric study.

    Neurology·2009
    Same author

    Antioxidant effect of ethyl pyruvate in respiring neonatal cerebrocortical slices after H(2)O(2) stress.

    Neurochemistry international·2008
    Same author

    Photoluminescent characterization of atomic diffusion in core-shell nanoparticles.

    Optics express·2008
    Same author

    NMR determination of oligonucleotide structure.

    Current protocols in nucleic acid chemistry·2008
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Supercoiled DNA exhibits exceptionally clear proton NMR spectra, revealing high mobility and structural modifications in A.T base pairs. This contrasts sharply with linear DNA, offering new insights into DNA dynamics.

    Area of Science:

    • Molecular Biology
    • Biophysics
    • Nuclear Magnetic Resonance Spectroscopy

    Background:

    • Proton Nuclear Magnetic Resonance (NMR) spectroscopy is a powerful tool for studying DNA structure and dynamics.
    • Supercoiled DNA, prevalent in biological systems, exhibits unique structural and dynamic properties compared to linear DNA.

    Purpose of the Study:

    • To investigate the proton NMR spectra of an intact supercoiled plasmid DNA molecule.
    • To characterize the mobility and structural features of supercoiled DNA using NMR.

    Main Methods:

    • Proton NMR experiments were conducted on a 7,200 base pair supercoiled plasmid DNA (pIns36) at 240 and 360 MHz.
    • Analysis focused on exchangeable imino protons and nonexchangeable aromatic protons.

    Main Results:

    Related Experiment Videos

    • Supercoiled DNA displayed remarkably well-resolved proton NMR spectra, unlike broadened signals in comparable linear DNA.
    • Observed linewidths suggest high molecular mobility, consistent with previous 31P NMR findings.
    • Chemical shift alterations in imino proton resonances indicate structural modifications in A.T base pairs, with at least two distinct structures apparent.

    Conclusions:

    • Supercoiled DNA exhibits significantly enhanced spectral resolution and mobility compared to linear DNA.
    • NMR data reveal structural heterogeneity in A.T base pairs within supercoiled DNA, while G.C base pairs appear largely unaffected.