Related Experiment Video

Updated: Aug 17, 2026

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
07:04

Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation

Published on: September 16, 2018

Modulatory effect of hydrocortisone on T-lymphocyte regulatory activity in pokeweed mitogen-driven Ig production

J A Brieva, E G de la Concha, D Pascual-Salcedo

    Clinical Immunology and Immunopathology
    |February 1, 1983
    PubMed

    The effect of hydrocortisone (HCO) on pokeweed mitogen (PWM)-driven Ig production from human tonsil B- plus T-lymphocyte cocultures was analyzed. IgM and IgG production decreased in cultures with a B/T ratio of 10/1 or higher (P less than 0.001) and increased in cultures with a B/T ratio of 1/1 or lower (P less than 0.01) when 10(-5) M HCO was added. Similar effects were observed on Ig production from peripheral blood lymphocyte cultures. These effects were only obtained at the HCO doses of 10(-5) and 10(-6) M, and no modifications by monocyte depletion were observed. The function of HCO-pretreated B cells did not differ from that of control B cells when cocultured with fresh T cells. However, the HCO pretreatment of T cells before coculturing with fresh B cells affected the Ig production in the same way as the addition of the drug to fresh cocultures. These results suggest that HCO modulates the PWM-driven Ig production by affecting helper and suppressor T-cell activity.

    More Related Videos

    Murine Model of Epicutaneously-Induced Immunomodulation09:07

    Murine Model of Epicutaneously-Induced Immunomodulation

    Published on: June 24, 2025

    A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes11:34

    A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

    Published on: April 11, 2025

    Related Concept Videos

    Inflammatory Response01:28

    Inflammatory Response

    An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
    Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
    T Cell Types and Functions01:24

    T Cell Types and Functions

    When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
    Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
    B Cell Activation and Differentiation01:24

    B Cell Activation and Differentiation

    The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
    When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...