A three-stage chromatographic procedure for cordycepin. Quantitative estimation in rat brain tissue

A technique for the quantitative estimation of intraperitoneally injected unlabelled cordycepin in the acid soluble pool (ASP) isolated from rat brain tissue has been suggested. It consists of consecutive chromatography of ASP on columns of Dowex 1 X 8, Dihydroxyboryl-SP500 and Sephasorb-HP. As a result, the fraction containing 2'-deoxyriboadenosine and 3'-deoxyriboadenosine (cordycepin) was isolated from the brain tissue ASP of experimental animals which were given a cordycepin injection. The 2'-deoxyriboadenosine fraction was isolated from tissue ASP of control animals which were not given an injection of this antibiotic. Brain tissue antibiotic content was estimated by the difference in mumolar nucleosides quantity values in the two fractions (control/experiment).