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[Poor fibrinolytic response to venous occlusion: defect of endothelial cells or plasma interference?]
To better characterize the plasminogen activators (v-PA) before and after venous occlusion, the euglobulins obtained from the plasma of 24 volunteers have been separated on SDS-PAGE and revealed zymographically. All the subjects with an excellent fibrinolytic response on fibrin plates (greater than 1.3 TAU/ml) exhibited activity of 68 kdaltons, which corresponds to free v-PA. The poor responders on fibrin plates (less than or equal to 0.3 TAU/ml) only showed increase of a lysis zone at 110 kdaltons, corresponding to a complex of v-PA and a fast-acting inhibitor. Hence poor fibrinolytic activity on fibrin plates after venous occlusion could be due to high plasma concentration of this inhibitor and not necessarily to defective production or release of v-PA.
To better characterize the plasminogen activators (v-PA) before and after venous occlusion, the euglobulins obtained from the plasma of 24 volunteers have been separated on SDS-PAGE and revealed zymographically. All the subjects with an excellent fibrinolytic response on fibrin plates (greater than 1.3 TAU/ml) exhibited activity of 68 kdaltons, which corresponds to free v-PA. The poor responders on fibrin plates (less than or equal to 0.3 TAU/ml) only showed increase of a lysis zone at 110 kdaltons, corresponding to a complex of v-PA and a fast-acting inhibitor. Hence poor fibrinolytic activity on fibrin plates after venous occlusion could be due to high plasma concentration of this inhibitor and not necessarily to defective production or release of v-PA.