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HLA-B27, ankylosing spondylitis and polymorph motility.

C T Pease, H L Currey

    British Journal of Rheumatology
    |November 1, 1983
    PubMed
    Summary

    Polymorphonuclear leucocyte function, specifically chemotaxis, was elevated in patients with ankylosing spondylitis (AS) and those positive for HLA-B27. This indicates a link between AS, HLA-B27 positivity, and enhanced immune cell migration.

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    Area of Science:

    • Immunology
    • Rheumatology
    • Human Genetics

    Background:

    • Ankylosing spondylitis (AS) is a chronic inflammatory disease primarily affecting the axial skeleton.
    • The human leukocyte antigen HLA-B27 is strongly associated with the development of AS.
    • Polymorphonuclear leucocyte (PMN) function, including chemotaxis, plays a crucial role in inflammatory responses.

    Purpose of the Study:

    • To investigate polymorphonuclear leucocyte (PMN) function in patients with ankylosing spondylitis (AS).
    • To determine if HLA-B27 positivity influences PMN chemotaxis in AS patients and controls.
    • To compare PMN chemotaxis in AS patients and healthy controls with varying HLA-B27 status.

    Main Methods:

    • Assessed polymorphonuclear leucocyte (PMN) functions in 29 AS patients (20 HLA-B27 positive, 9 negative) and 30 healthy controls (15 HLA-B27 negative).
    • Measured random and directed cell migration (chemotaxis) using micropore filter and under-agar assays.
    • Utilized serum activated by casein or zymosan as the chemoattractant for PMN migration.

    Main Results:

    • Directed cell migration (chemotaxis) was significantly increased in AS patients compared to controls.
    • Subjects positive for HLA-B27, irrespective of AS diagnosis, exhibited enhanced PMN chemotaxis.
    • Both AS and HLA-B27 positivity were independently associated with increased chemotaxis.

    Conclusions:

    • The study suggests that enhanced PMN chemotaxis is a feature associated with ankylosing spondylitis.
    • HLA-B27 positivity appears to be linked to an intrinsic increase in PMN chemotactic ability.
    • These findings may contribute to understanding the pathogenesis of AS and the role of HLA-B27 in immune cell function.

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