In vitro transformation of rat brain cells by adenovirus type 12

Whole brain cells, taken from Sprague-Dawley rats of various ages: 13-, 14-, and 16-day fetuses, and 2- and 13-day-old animals were transformed in vitro by adenovirus type 12 (Ad. 12). Immature or dedifferentiated astrocytes (As cells), neurons, and ependymal cells could clearly be identified by conventional staining methods and scanning electron microscopy. Ependymoglioblast-like cells, oligodendrocytes, and undifferentiated cells were also recognized. Sequential observation disclosed that a probable candidate for the target of transformation by Ad. 12 might be As cell which was the most predominant cell type. The transformation rate was highest in the secondary cultures from 16-day fetuses and lowest in the secondary cultures from 13-day-old animals.