[Purification and immunochemical characteristics of a specific membrane protein from human brain]
Abstract:
Three specific antigens, PS-1, PS-2 and PS-3 were identified by crossed immunoelectrophoresis. The proteins were solubilized from adult human brain crude membrane fraction with Triton X-100 and purified by column chromatography on phenyl-Sepharose CL 4B. The PS-3 antigen was shown to be identical to protein D2. Antigen PS-2 was purified by affinity column chromatography consecutively with phenyl-Sepharose CL 4B, concanavalin Sepharose 4B, lysin-Sepharose and phytohemagglutinin-Sepharose. The tissue specificity of the protein was characterized by rocket-line immunoelectrophoresis of the extracts from various human tissues in interpose gel. Protein PS-2 was detected in fetal human brain tissue in large amounts. The fetal protein form did not absorb on phenyl-Sepharose. PS-2 is an interspecies brain protein which gives a criss-cross immunochemical reaction with ox and rat brain extracts. Protein PS-2 content is 30% of the total protein content of adult human brain crude membrane fraction.
