[Preservation of ram seminal fluid in different diluents]

Studied was the conformation of the protein macromolecules with the dilution of ram semen to a various extent, using three yolk diluents -- gkh18, zhgts, and Zlatno rouno. Studied was also the effect of some yolk extenders used in this country's practice on some qualitative indices of ram semen under storing conditions. It was found that following dilution with the media cited above various conformational changes of the proteins take place with the semen of rams. The different rates of dilution had no effect on the primary motility of spermatozoa. Statistically significant was the difference in favour of the gkh18 diluent in so far as the heat resistance of spermatozoa was concerned. Most effective dilution (in terms of retaining the motility of spermatozoa at storing) proved 1 + 3, followed by 1 + 5 with the use of gkh18, and 1 + 1 with zhgts and Zlatno rouno. gkh18 was shown to produce a varying blocking effect on motility of spermatozoa. All results concerning the conformation of protein macromolecules, quality indices, and particularly the resistance to heat in evaluating the storing capacity of semen showed that the cumulation of more proteins of higher electrophoretic mobility in III group developed more favourable conditions in preserving the vitality of spermatozoa at storage.