Isolation and analysis of odorous components in swine manure

Systematic procedures are described for the isolation and extraction of odorous components in swine faeces, urine and rotten mixtures of swine faeces and urine. Samples were frozen and subjected to vacuum distillation in the frozen state. The distillate was continuously extracted with diethyl ether. The residue was extracted with diethyl ether and the extract was subjected to vacuum distillation. The former extract and the latter distillate were combined and concentrated. Recovery by these procedures was considered. Odorous compounds isolated were analyzed by gas chromatography and gas chromatography-mass spectrometry.