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Study of urinary specific gravity by reagent strip method.

K Ito, M Niwa, T Koba

    The Tokai Journal of Experimental and Clinical Medicine
    |July 1, 1983
    PubMed
    Summary
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    A new reagent strip accurately measures urinary specific gravity by detecting cation concentrations. This convenient method correlates well with traditional techniques and is suitable for routine urinalysis.

    Area of Science:

    • Clinical Chemistry
    • Urinalysis

    Background:

    • Urinary specific gravity is a key indicator of kidney function and hydration status.
    • Traditional methods for measuring specific gravity include refractometry and gravimetry, which can be time-consuming.
    • A need exists for rapid, accurate, and convenient methods for routine urinalysis.

    Purpose of the Study:

    • To describe a newly developed reagent strip method for determining urinary specific gravity.
    • To evaluate the accuracy and reliability of this new method compared to established techniques.

    Main Methods:

    • A novel reagent strip (Chemical S.G., Ames Co.) utilizing polyelectrolyte sensitive to cations was employed.
    • Measurements were compared with those obtained by refractometry and gravimetry of NaCl solutions.
    • Correlation with urinary osmolarity was also assessed.

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    Main Results:

    • The reagent strip measurements correlated well with refractometry (r = 0.79-0.93) and gravimetry.
    • Unlike refractometry, the reagent strip was unaffected by urinary glucose and urea concentrations.
    • The method demonstrated good reproducibility and accuracy.

    Conclusions:

    • The developed reagent strip offers a convenient and accurate tool for routine urinalysis.
    • This method provides reliable urinary specific gravity measurements, unaffected by common urinary solutes other than cations.
    • The ease of handling and good performance make it suitable for clinical laboratory settings.