RNA metabolism in M1 adrenergic neuroblastoma cells

RNA metabolism of M1 adrenergic neuroblastoma cells was investigated in logarithmic proliferating, stationary and differentiated states. [3h] uridine labelling experiments showed that in the stationary phase (10 days of culture) the cells incorporated less radioactive precursors into RNA than in the logarithmic phase (3 days of culture). Cells differentiated by bromodeoxyuridine treatment were compared with proliferating cells. At short labelling time, the BrdU-treated cells incorporated more [3H] uridine in RNA than the controls. But when morphological differentiation became irreversible, the treated cells incorporated less [3H] uridine into RNA. Our results show that morphological differentiation by BrdU-treatment of neuroblastoma M1 cells is not accompanied by striking changes in RNA metabolism.