Steroid profiles of body fluids other than urine, obtained by capillary gas chromatography

Steroid profiling by capillary gas chromatography has been applied to the analysis of free and conjugated steroids in serum, ovarian follicles, lutein cyst and peritoneal fluids. Sep-Pak C18 octadecylsilica was used for extraction, whereas the separation of free and different conjugates was accomplished by successive hydrolysis and liquid extraction steps.