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Updated: Aug 7, 2026

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches
Published on: May 6, 2015
DNA-binding domain in adenovirus core protein VII
Abstract:
Major core protein, VII of adenovirus type 2 can be cleaved with BrCN into four fragments A, B, C and D according to the electrophoretic mobility on SDS-polyacrylamide gel. The sequential order of the fragments was ACBD from NH2-terminus to COOH-end. From the results of protein blotting experiment and nitrocellulose filter binding assay, fragments A and C, the amino end half of VII, was suggested to be DNA-binding domain. Furthermore, the binding of VII to DNA is suggested to become tighter as the fragment A moiety of pVII is processed in the virion.
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