Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simplified method to determine binding parameters in a two-site case using linear regression.

V Guardabasso, F Benfenati

    Computer Programs in Biomedicine
    |December 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Graphene oxide increases the phototransduction efficiency of copolymeric nanoimplants and rescues visual functions in rat and pig models of Retinitis pigmentosa.

    Nature communications·2025
    Same author

    Spiropyran-based glutamate nanovalve for neuronal stimulation.

    Materials horizons·2025
    Same author

    Solid-State Nanopores for Spatially Resolved Chemical Neuromodulation.

    Nano letters·2024
    Same author

    Light-induced charge generation in polymeric nanoparticles restores vision in advanced-stage retinitis pigmentosa rats.

    Nature communications·2022
    Same author

    Organic semiconductors for artificial vision.

    Journal of materials chemistry. B·2020
    Same author

    Synapsin I Synchronizes GABA Release in Distinct Interneuron Subpopulations.

    Cerebral cortex (New York, N.Y. : 1991)·2019
    Same journal

    Microprocessor-based system for on-line analysis of respiratory responses to exercise.

    Computer programs in biomedicine·1985
    Same journal

    A calculator program for clinical application of the Bayesian method of predicting plasma drug levels.

    Computer programs in biomedicine·1985
    Same journal

    Testing marginal homogeneity in square tables; with emphasis on matched data.

    Computer programs in biomedicine·1985
    Same journal

    Computerized analysis of enzyme cascade reactions using continuous rate data obtained with an ELISA reader.

    Computer programs in biomedicine·1985
    Same journal

    Mathematical modelling of stimulus-secretion coupling in the pancreatic B-cell. II. Calcium-stimulated calcium release.

    Computer programs in biomedicine·1985
    Same journal

    A program for logistic prediction modelling.

    Computer programs in biomedicine·1985
    See all related articles

    This study presents a simplified iterative program to estimate binding parameters for systems with two independent binding sites. The method is suitable for microcomputers and programmable calculators, offering a practical approach to analyzing binding data.

    Area of Science:

    • Biochemistry
    • Pharmacology
    • Analytical Chemistry

    Background:

    • Accurate estimation of binding parameters is crucial for understanding molecular interactions.
    • Traditional methods for analyzing binding data can be complex, especially for systems with multiple binding sites.
    • Scatchard and Hofstee plots are common graphical methods for visualizing binding data.

    Purpose of the Study:

    • To present a simplified computational method for estimating binding parameters.
    • To develop a program applicable to systems with two independent classes of binding sites.
    • To facilitate the analysis of binding data using accessible computing tools.

    Main Methods:

    • An iterative approach is employed to approximate binding activities.
    • The method starts from curvilinear Scatchard or Hofstee plots.

    Related Experiment Videos

  • The program is designed for microcomputers with graphic capabilities and programmable calculators.

    • Main Results:

    • The program provides estimates of binding parameters for two independent binding sites.
    • The iterative method simplifies the step-by-step approximation of binding lines.
    • Successful application on microcomputers and calculators demonstrates practicality.

    Conclusions:

    • The presented simplified method offers an efficient way to compute binding parameters.
    • This approach enhances the accessibility of binding data analysis.
    • The program's design supports its use in various laboratory settings with limited computational resources.