A simple device for the collection of cells sorted by flow cytometry

Area of Science:

• Biotechnology
• Cell Biology
• Analytical Chemistry

Background:

• Flow cytometry is a powerful technique for cell analysis and sorting.
• Effective cell collection post-sorting is crucial for downstream applications.
• Current methods may compromise cell viability and morphology.

Purpose of the Study:

• To describe a simple device for collecting cells sorted by flow cytometry.
• To evaluate the device's performance under both sterile and nonsterile conditions.
• To assess the recovery rate, viability, and cytomorphology of collected cells.

Main Methods:

• Development of a novel cell collection device for flow cytometry.
• Testing the device under sterile and nonsterile laboratory conditions.
• Analysis of cell recovery, viability, and cytomorphology post-collection.

Main Results:

• The described device enables efficient cell collection following flow cytometry sorting.
• High recovery rates of sorted cells were achieved.
• Superior preservation of cell viability and cytomorphology was observed.

Conclusions:

• The developed device offers a simple yet effective solution for cell collection after flow cytometry.
• It ensures high-quality cell samples suitable for further analysis.
• The device is applicable in both sterile and nonsterile experimental settings.