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Pock-forming plasmids isolated from streptomyces roseochromogenus.
The Journal of Antibiotics
|May 1, 1984
Summary
Researchers isolated high copy number plasmids (pSRC) from Streptomyces, demonstrating their self-transmissibility and wide host range. These pSRC plasmids show potential as versatile cloning vectors for Streptomyces research.
Area of Science:
- Molecular Biology
- Microbial Genetics
- Biotechnology
Background:
- Plasmids are crucial tools in genetic engineering, particularly in microbial systems.
- Streptomyces species are known for producing valuable secondary metabolites and are important hosts for genetic manipulation.
Purpose of the Study:
- To isolate and characterize novel high copy number plasmids from Streptomyces roseochromogenus S264.
- To evaluate the potential of these plasmids as cloning vectors in Streptomyces.
Main Methods:
- Isolation of plasmids from Streptomyces roseochromogenus S264.
- Characterization of plasmid properties including self-transmissibility and lethal zygosis (Ltz) phenotype.
- Determination of host range via conjugation.
- Cloning of the thiostrepton resistance gene into pSRC1b to identify restriction sites.
Main Results:
- A series of high copy number plasmids, designated pSRC, were successfully isolated.
- pSRC plasmids exhibit self-transmissibility and a wide host range, detected using the Ltz phenotype.
- pSRC1 comprises two plasmids (pSRC1a and pSRC1b); pSRC1b possesses a non-essential Bgl II site suitable for DNA insertion.
Conclusions:
- The isolated pSRC plasmids possess desirable characteristics for genetic manipulation in Streptomyces.
- The identified restriction sites and replication capabilities suggest the pSRC series' suitability as novel cloning vectors.