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Updated: Aug 17, 2026
Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
Published on: November 24, 2010
[Microbiological and immunodiagnostic studies in experimental erysipeloid]
Abstract:
The use of the passive hemagglutination test with a set of antigenic and antibody erythrocytic diagnostic reagents made it possible to detect antibodies to Erysipelothrix rhusiopathiae specific and type specific antigens in the blood serum of infected mice and to reveal the presence of these antigens in the internal organs of the animals. After a considerable increase of the infective dose a higher level of contamination and the retention of the antigens in the internal organs were observed, while the activity of specific antibodies in the blood serum decreased.
Insights
The passive hemagglutination test detected Erysipelothrix rhusiopathiae antibodies and antigens in mice. Higher infection doses increased antigen presence but decreased antibody levels.
Area of Science:
- Veterinary Microbiology
- Immunology
Background:
- Erysipelothrix rhusiopathiae causes significant disease in animals.
- Accurate diagnostic methods are crucial for controlling E. rhusiopathiae infections.
Purpose of the Study:
- To evaluate the passive hemagglutination test for detecting E. rhusiopathiae antibodies and antigens.
- To investigate the relationship between infection dose, antigen distribution, and antibody response.
Main Methods:
- Passive hemagglutination test using specific antigenic and antibody erythrocytic reagents.
- Analysis of blood serum and internal organs of infected mice.
Main Results:
- The passive hemagglutination test successfully detected antibodies to E. rhusiopathiae specific and type-specific antigens in infected mice.
- Antigens were identified in the internal organs of infected animals.
- Increased infective dose led to higher contamination levels and antigen retention in organs, concurrently decreasing specific antibody activity in serum.
Conclusions:
- The passive hemagglutination test is a viable method for diagnosing E. rhusiopathiae infections.
- Infection dynamics involve antigen persistence in organs and a complex antibody response, influenced by the infective dose.
