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A simultaneous assay to quantitate plasma and endometrial hormone concentrations
Fertility and Sterility
|July 1, 1978
Summary
This study presents a radioimmunoassay method to measure hormones in plasma and endometrium. Hormone levels in the endometrium correlate with circulating hormone levels, aiding in understanding hormone effects on target tissues.
Area of Science:
- Endocrinology
- Reproductive Biology
- Biochemistry
Background:
- Hormonal fluctuations significantly impact reproductive health.
- Understanding the relationship between circulating hormones and target tissue concentrations is crucial for reproductive research.
Purpose of the Study:
- To present a validated radioimmunoassay (RIA) method for simultaneous determination of hormones in plasma and endometrial tissue.
- To investigate the correlation between plasma and endometrial concentrations of total estrogen (TE) and progesterone (P) throughout the menstrual cycle.
Main Methods:
- Simultaneous measurement of TE and P in plasma and endometrium from 59 women using RIA.
- Analysis of hormone concentrations across different phases of the menstrual cycle.
Main Results:
- Endometrial TE peaked at midcycle (4.89 ng/gm) and was constant during the secretory phase (2.2 ng/gm).
- Endometrial P concentrations were 5.31 ng/gm (proliferative) and 44.93 ng/gm (luteal).
- High correlation coefficients (0.8 for TE, 0.9 for P) were observed between plasma and endometrial hormone levels.
Conclusions:
- The developed RIA method accurately reflects circulating hormone levels in endometrial tissue.
- These findings support the utility of simultaneous hormone measurements in studying the role of hormones in local biological phenomena.