Prevention of glucose-induced insulin secretion by lipoxygenase inhibitor

Glucose-induced insulin secretion was investigated using isolated pancreatic islets of rats. The phospholipase A2 inhibitors. p-bromophenacyl bromide (0.1 mM) and mepacrine (0.1 mM) inhibited glucose-induced insulin secretion. Indomethacin (5 microM), a cyclooxygenase inhibitor, failed to inhibit glucose-induced insulin secretion, while the lipoxygenase inhibitor nordihydroguaiaretic acid (0.1-0.2 mM) inhibited it. These results suggest that stimulation of phospholipase A2 and a product(s) formed by the lipoxygenase pathway play an important role in the glucose-induced insulin secretion.