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Optimized PCR-based Detection of Mycoplasma
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Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

Simple method for quantitation of viable mycoplasmas

A C Albers, R D Fletcher

    Applied and Environmental Microbiology
    |April 1, 1982
    PubMed

    Abstract:

    A rapid, simple, and inexpensive method for quantitation of viable mycoplasmas is described. Serial dilutions were made in sterile microtiter plates with standard microtiter equipment. The results were multiplied by a factor of 2.38 to obtain colony-forming units comparable to those obtained with the laborious glass pipette-tube dilution method.

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