[Production of monospecific type A botulinum toxin and antiserum using a column chromatographic method]

A column chromatography method was employed to obtain a monospecific botulinus type A toxin. The latter was found to be a homogeneous product via column chromatographic and electrophoretic studies. The immunodiffusion test after Ouchterlony with a serum obtained with the use of a native toxin yielded one precipitation line. No such lines were produced with a serum obtained from animals immunized with a type B toxin. The purified toxin could be detoxicated by routinely applied methods. When injected to rabbits it induced the production of monospecific antibodies.