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Updated: Aug 11, 2026

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
10:33

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

[Isolation of anti-foot and mouth IgG by means of affinity chromatography]

N Marchev, E Veleva, N Bazhlekov

    Veterinarno-Meditsinski Nauki
    |January 1, 1979
    PubMed

    The coupling of the F. M. D. antigen with unsoluble CNBr-activated sepharose (2B or 4B) induced the production of an active agent with which the specific F. M. D. antibody of the investigated solution may covalently couple. Coupling procedures prove most effective with the use of buffer solutions of high ion strength that is necessary for the reduction of a protein-protein adsorption initiated by the polyoelectrolite nature of proteins. The produced complexes of the type CNBr activated sepharose--12C antigen--antibody after elimination of the unspecific serum proteins are dissociated up to the release of a specific IgG antibody.

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