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[Mathematical determination of the growth curve parameters of BHK-21(C13) multiplying in suspension culture]
Abstract:
A mathematical method for nomination of the continuity of the growing curve BHK-21(C13) phases, cultivated in the conditions of a one litre fermentor was used. The number of cell dividing (n), the speed of dividing (R) and the time of the entire dividing of the culture were calculated as well. It was established that the lowest point of the growing curve was in the 4th h and 34th min from the beginning of cultivation, the duration of the exponential phase is 20 h and 15 min. At the end of the exponential phase n is 1.85, Tg--12 h, 57 min and R--0.077. The short lag-phase is explained with the three dimension cell multiplication.
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