[Production on alkaline protease by Bacillus subtilis NRRL 3411]

The aim of this work was to study the influence of medium composition and the effect of the operative conditions on alkaline protease production. Several nitrogen sources (in amount of 10 g/l) were tested and compared with bacto peptone Difco: Casein was the best. The addition of "Tween 80" 0,5% resulted in a marked increase in yields of the alkaline protease. The maximum alkaline protease production (3.342.290 UAPAM/g) was achieved using the following medium: Lactose 20 g/l; casein 10 g/l; NaCl 1,5 g/l; MgSO4.7H2O 0,15 g/l; CaCl2.2H2O 0,06 g/l; MnCl2.4H2O 0,01 g/l; "Tween 80" 5,0 g/l; EDTA 1,5 X 10(-4)M; KH2PO4 1,5 g/l; K2H PO4 1,5 g/l; Na2SO4 1,5 g/l. It was found that a volumetric relation of 0,1 ml, vol. of medium/vol. of flask at 25 degrees C of temperature was the best process condition.