Chromosomal aberrations in foreign body tumorigenesis of mice

Sarcomas were induced in 107 male and female isogeneic CBA/H or CBA/H-T6 mice by subcutaneous implantation of double films of unplasticized vinylchloride-acetate copolymer, 15 x 22 x 0.2 mm in size. Tumors were grouped by chromosome number. G-banding was performed on chromosomes of (a) 12 sarcomas, (b) 6 specimens of preneoplastic cells derived from foreign body (FB)-reactive tissues at 4, 6, 9, and 16 months postimplantation, and (c) 11 sarcomas which developed from clonal lines of the preneoplastic cells studied. Karyological analyses lead to the following results and conclusions: (1) Various derangements in chromosome number occurred in preneoplastic cells during early FB reaction at the time of, and possibly in causal relation to carcinogenic initiation. (2) Structural abnormalities of specific chromosomes (insertions, translocations, transpositions, etc.) were found as stable cell markers only during late preneoplasia. They may thus contribute to advanced tumor progression. (3) Ploidy deviations of specific chromosomes (secondary to the early derangements in chromosome number) were most frequently seen in chromosomes 1, 6, 7, 13, 15, 18, and 19; however, these latter aberrations were unstable and inconsistent both in vivo and in vitro.