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The E-rosette augmenting factor (E-RAF): aspects of clinical application

A I Agbata, C H Kirkpatrick

    Clinical and Experimental Immunology
    |February 1, 1980
    PubMed
    Summary
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    Normal lymphocytes release a soluble E-rosette augmenting factor (E-RAF) that enhances E-rosette formation. This factor

    Area of Science:

    • Immunology
    • Cellular Immunology

    Background:

    • Antigen- or mitogen-stimulated lymphocytes release a soluble E-rosette augmenting factor (E-RAF).
    • E-RAF enhances E-rosette formation, a process involving lymphocyte-red blood cell interactions.
    • E-rosette formation can serve as an immunologic marker.

    Purpose of the Study:

    • To investigate the release of E-RAF and its effect on E-rosette formation in normal volunteers and patients with immune deficiencies.
    • To evaluate the utility of E-rosette formation as an assay for lymphocyte function.

    Main Methods:

    • Studied lymphocytes from normal volunteers, patients with chronic mucocutaneous candidiasis, and a patient with severe combined immune deficiency (SCID).
    • Stimulated lymphocytes with mitogens (PHA, Con A) or specific antigens.

    Related Experiment Videos

  • Measured E-rosette formation as an indicator of E-RAF release and lymphocyte response.
  • Assessed short-term incubation of normal lymphocytes in mitogen-derived supernatant.

    • Main Results:

    • Lymphocytes from normal donors released E-RAF and showed increased E-rosette formation upon stimulation.
    • Lymphocytes from the SCID patient failed to release E-RAF and increase E-rosette formation after mitogenic stimulation.
    • Short-term incubation of normal lymphocytes in E-RAF-containing supernatant proved to be a reliable assay for total E-rosettes.

    Conclusions:

    • Confirms the release of E-RAF by stimulated normal lymphocytes and its role in enhancing E-rosette formation.
    • Highlights the impaired E-RAF release and E-rosette formation in SCID, indicating a defect in lymphocyte function.
    • Proposes a reliable assay for total E-rosettes using E-RAF and short-term lymphocyte incubation.