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Enzyme therapy and immuno-adsorption by an extra-corporeal device
Biomaterials, Medical Devices, and Artificial Organs
|January 1, 1980
Summary
A novel extracorporeal chamber offers safe and effective hemoperfusion for various medical treatments, including tumor antigen isolation and L-asparaginase therapy. This innovative device shows promise in managing hypernephroma, lymphoma, hemophilia, and kidney transplant rejection without adverse effects.
Area of Science:
- Biomedical Engineering
- Immunology
- Oncology
Background:
- Extracorporeal therapies are crucial for managing various diseases.
- A need exists for versatile and safe devices for hemoperfusion, enzyme therapy, and immunoadsorption.
Purpose of the Study:
- To develop and evaluate a novel extracorporeal chamber for diverse therapeutic applications.
- To assess the safety and efficacy of the chamber in isolating tumor antigens, administering L-asparaginase, and reducing antibodies.
Main Methods:
- Construction of a polymethyl-acrylate extracorporeal chamber (10x18x7 cm) with 23 plates (0.5 m² surface area).
- Covalent binding of active substances to plate surfaces, enabling hemoperfusion (3-6 hr) typically without a pump.
- Application of immobilized homologous antisera (F(ab')2) for antigen isolation and L-asparaginase for therapy.
Main Results:
- Successfully isolated three putative tumor-associated antigens from hypernephroma cases.
- Achieved tumor remissions in lymphoma patients treated with extracorporeal L-asparaginase.
- Significantly reduced Factor VIII antibodies in hemophilia patients via immunoadsorption.
- Prevented rejection in kidney transplant recipients using L-asparaginase therapy.
- Treated approximately 50 patients without observed infections, thrombocyte decrease, or other side effects.
Conclusions:
- The developed extracorporeal chamber is a safe and effective platform for hemoperfusion, enzyme therapy, and immunoadsorption.
- The device demonstrates broad applicability in oncology, immunology, and transplantation medicine.
- Further exploration of its use in other diseases is warranted.