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Lactate dehydrogenase in rabbit oocytes and eggs
Canadian Journal of Genetics and Cytology. Journal Canadien De Genetique Et De Cytologie
|June 1, 1978
Summary
Rabbit oocyte lactate dehydrogenase (LDH) isozyme patterns do not change at ovulation. Follicle cells, not oocytes, cause apparent LDH shifts, resolving conflicting literature findings.
Area of Science:
- Reproductive biology
- Biochemistry
Background:
- Recent reports suggest a significant shift in rabbit oocyte lactate dehydrogenase (LDH) isozyme patterns during ovulation.
- This reported shift has raised questions about oocyte maturation and function.
Purpose of the Study:
- To investigate the reported shift in rabbit oocyte lactate dehydrogenase (LDH) isozyme patterns at ovulation.
- To clarify the true LDH isozyme profile of oocytes and ovulated eggs and identify potential sources of experimental variability.
Main Methods:
- Lactate dehydrogenase (LDH) isozyme analysis was performed on ovarian oocytes and ovulated eggs.
- Experiments were conducted both with and without the removal of surrounding follicle cells.
- LDH isozyme patterns of isolated follicle cells were also analyzed.
Main Results:
- Both ovarian oocytes and ovulated eggs predominantly exhibit LDH-5, with a minor presence of LDH-4.
- No significant change in LDH isozyme pattern was observed between oocytes and ovulated eggs.
- When follicle cells were not removed, all five LDH isozymes were detected.
- Isolated follicle cells themselves displayed all five LDH isozymes.
Conclusions:
- The rabbit oocyte lactate dehydrogenase (LDH) isozyme pattern remains consistent from the ovarian oocyte stage through ovulation.
- The presence of follicle cells can artifactually alter observed LDH isozyme patterns.
- Failure to remove follicle cells likely explains previously reported conflicting results in the literature regarding rabbit oocyte LDH isozymes.