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Related Experiment Videos

Small-size mRNAs code for ribosomal proteins in yeast

G H Bollen, W H Mager, L W Jenneskens

    European Journal of Biochemistry
    |March 1, 1980
    PubMed
    Summary

    Researchers isolated messenger RNA (mRNA) encoding yeast ribosomal proteins. This mRNA fraction, approximately 9S, successfully directed the synthesis of small, basic proteins characteristic of ribosomal proteins in vitro.

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    Area of Science:

    • Molecular Biology
    • Yeast Genetics
    • Protein Synthesis

    Background:

    • Understanding ribosomal protein gene function is crucial for studying protein synthesis.
    • Purification of specific messenger RNA (mRNA) is a key step in gene identification.

    Purpose of the Study:

    • To identify and characterize mRNA molecules that code for ribosomal proteins in yeast.
    • To develop a method for isolating and utilizing these specific mRNA fractions.

    Main Methods:

    • Polyadenylated RNA from Saccharomyces carlsbergensis was isolated and fractionated by size using sucrose gradient centrifugation.
    • Size-fractionated mRNA was translated in a cell-free wheat germ system.
    • In vitro synthesized proteins were analyzed using various gel electrophoresis techniques, including SDS-PAGE and 2D gel electrophoresis.

    Main Results:

    • A specific mRNA fraction, approximately 9S in size, was found to direct the synthesis of proteins with characteristics of ribosomal proteins (small and basic).
    • Two-dimensional gel electrophoresis confirmed the ribosomal nature of the synthesized proteins.
    • This purified 9S mRNA fraction serves as a valuable probe for identifying ribosomal protein genes.

    Conclusions:

    • A distinct mRNA fraction (9S) responsible for synthesizing yeast ribosomal proteins was successfully isolated.
    • This purified mRNA provides a tool for the molecular cloning and study of yeast ribosomal protein genes.

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