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Application of mutagenicity test for milk

M Green, E Ben-Hur, E Riklis

    Journal of Dairy Science
    |March 1, 1980
    PubMed
    Summary

    Mutagenic activity was detected in heat-sterilized milk using the Ames test, indicating potential DNA damage risks. This mutagenic compound was found in the milk

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    Area of Science:

    • Food safety and toxicology
    • Molecular biology and genetics

    Background:

    • Milk and dairy products are staple foods globally.
    • Assessing potential mutagenicity in food is crucial for public health.
    • Previous studies on milk mutagenicity are limited.

    Purpose of the Study:

    • To investigate the presence of mutagenic activity in milk and milk products.
    • To characterize the mutagenic potential using the Salmonella mammalian-microsome mutagenicity test (Ames test).

    Main Methods:

    • Utilized the Salmonella mammalian-microsome mutagenicity test (Ames test).
    • Tested heat-sterilized milk samples.
    • Employed the S-9 fraction of microsomes for metabolic activation.
    • Analyzed chloroform extracts of milk for mutagenic compounds.

    Main Results:

    • Positive mutagenic results were observed in heat-sterilized milk, particularly after S-9 activation.
    • A significant increase in His+ revertants (more than double the spontaneous rate) was recorded.
    • Mutagenic activity was detected in the chloroform extract of milk, suggesting a lipophilic nature.

    Conclusions:

    • Heat-sterilized milk contains mutagenic activity detectable by the Ames test.
    • The mutagenic compound requires metabolic activation for full expression.
    • Further research is needed to chemically identify the mutagenic substance in milk.

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