[Mechanism and means of eliminating induced luminescence in immunofluorescence]

The causes of the appearance of induced fluorescence are discussed. The classification of its different forms is proposed. A hypothesis on the role of cytophilic properties of fluorescent antibodies in the mechanism of induced nonspecific fluorescence is put forward. This hypothesis has been experimentally confirmed in the studies in the field of obtaining the Fab fragments of fluorescent antibodies and their approbation. Conclusion has been made concerning the good prospects and advisability of using the Fab fragments of antibodies as an immunofluorescent reagent; the Fab fragments of pure antibodies may be considered an ideal variant of such a reagent.