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Updated: May 8, 2026

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
Published on: March 15, 2011
The cytoplasmic filamentous network in cultured ovarian granulosa cells
Abstract:
We have utilized a cracking procedure followed by a rotary shadow technique to examine the ultrastructure of cultured ovarian granulosa cells. We have demonstrated that the structures observed are not artifacts of fixation or cracking by generating equivalent images following freezing and deep etching as well as by fixation prior to cracking. The cytoplasm of granulosa cells exhibits a complex cytoskeletal lattice composed of many 40-55 nm filaments. This filamentous network is continuous with the plasma membrane and appears to incorporate all formed elements within the cytoplasm. Filaments are organized in three ways: first, in large bundles, second, in individual filaments that are in direct association with organelles, and third, in a complex branching and anastomosing configuration. S-1 decoration revealed that the predominant filament species is action.
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