[Application of infrared spectroscopy to enterobacteria research]

The influence of technology used in the preparation of spectroscopic specimens on the intensity of analytical adsorption bands in the infrared spectra of intact microbial cells has been studied with the use of mathematical planning methods. The optimal technology of specimen preparation by KBr immersion has been selected. This technology ensures the measuring of the relative optical density of the main analytical adsorption bands with an error not exceeding 3%. As an example, several E. coli strains have been used to demonstrate the possibility of their differentiation by the infrared adsorption spectra of intact cells.