Parameters influencing the in vitro growth of Mycobacterium lepraemurium

Investigations were done on the role of several parameters of incubation, carbon sources, fatty acids and Tween 80 on the in vitro growth of M. lm on Ogawa medium. It was found that incubation of aerated tubes at 37 degree C in a humidified atmosphere was optimal. Glycerol is necessary in the medium at concentrations of 0.5 to 2%. Acetate inhibits growth; there is no beneficial effect from Tween 80 or sodium salts of oleic, caproic, caprylic and palmitic acid. The pH of the medium is very critical since optimal growth occurs only between pH 5.8 and 6.3 on Ogawa egg yolk medium.