Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prostacyclin is not a circulating hormone in man

I A Blair, S E Barrow, K A Waddell

    Prostaglandins
    |April 1, 1982
    PubMed
    Summary

    A new assay accurately measures 6-oxo-prostaglandin F1 alpha in plasma. This study found prostacyclin is not a circulating hormone in humans under normal conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Concise Guide to PHARMACOLOGY 2013/14: overview.

    British journal of pharmacology·2014
    Same author

    Intracellular drug concentrations.

    Clinical pharmacology and therapeutics·2013
    Same author

    Acute effects of varying doses of propranolol upon oxygen haemoglobin affinity in man.

    British journal of clinical pharmacology·2012
    Same author

    Metabolism of terbutaline in man and dog.

    British journal of clinical pharmacology·2012
    Same author

    Lapatinib-induced liver injury characterized by class II HLA and Gilbert's syndrome genotypes.

    Clinical pharmacology and therapeutics·2012
    Same author

    Chairman's Introduction.

    Proceedings of the Royal Society of Medicine·2010

    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Endocrinology

    Background:

    • Prostaglandins, like prostacyclin, play crucial roles in physiological processes.
    • Measuring plasma levels of prostaglandin metabolites is essential for understanding their function.
    • Previous methods for quantifying 6-oxo-prostaglandin F1 alpha had limitations in sensitivity and specificity.

    Purpose of the Study:

    • To develop and validate a highly specific assay for quantifying 6-oxo-prostaglandin F1 alpha in human plasma.
    • To determine the physiological levels of 6-oxo-prostaglandin F1 alpha in healthy volunteers.
    • To ascertain whether prostacyclin functions as a circulating hormone in humans.

    Main Methods:

    • Development of a stable isotope dilution assay.
    • Utilisation of capillary column gas chromatography.
    • Coupling with negative ion chemical ionisation mass spectrometry (NICIMS) for high sensitivity and specificity.

    Main Results:

    • The assay demonstrated a limit of sensitivity of 0.5 pg/mL.
    • Plasma concentrations of 6-oxo-prostaglandin F1 alpha in 20 healthy volunteers were all below 3 pg/mL.
    • These levels are significantly lower than previously reported values.

    Conclusions:

    • The developed assay is highly specific and sensitive for measuring plasma 6-oxo-prostaglandin F1 alpha.
    • The findings indicate that prostacyclin is not a circulating hormone in humans under normal physiological conditions.
    • This research provides critical data for understanding prostaglandin metabolism and hormonal regulation.

    Related Experiment Videos