Antibodies to sperm-associated antigens detected by solid phase assays

Solid phase assays using radiolabeled (RIA) or enzyme-linked (ELISA) antibodies are described for the quantitation of antibodies to sperm-associated antigens. Antibodies were detected in serum from rabbits immunized with human sperm emulsified in Freund's complete adjuvant and in the plasmas or sera of vasectomized males and patients with suspected antibody-mediated infertility. The RIA and ELISA assays are comparable to each other in sensitivity and specificity, rapid in execution, and inexpensive.