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Effect of surgery on monocyte function
Abstract:
In vitro monocyte lysozyme production (IVMLP) was assessed in 10 male patients preoperatively and at intervals postoperatively. A similar procedure was carried out in 6 sex- and age-matched controls. In contrast to the control group where the IVMLP was constant, the IVLMP levels in the surgical group showed a marked increase at 6-10 h postoperatively, falling to control levels after 10 days. In addition, peripheral monocyte counts were carried out using a Technicon Hemalog D counter which produces accurate reproducible counts for cells such as monocytes which occur at low frequency. The peripheral monocyte counts were stable in the control group, whilst in the surgical patients there were significantly higher readings at 36-48 h, which persisted for up to 10 days compared with preoperative counts. This study suggests that surgery has both a qualitative (IVMLP) and quantitative (monocyte counts) effect on the mononuclear phagocytes. These data may be relevant when considering factors that influence the early stages of wound healing.
Insights
Surgery impacts immune cells, increasing in vitro monocyte lysozyme production (IVMLP) and peripheral monocyte counts post-operation. These changes in mononuclear phagocytes may affect wound healing.
Area of Science:
- Immunology
- Surgical Science
Background:
- Mononuclear phagocytes play a crucial role in immune responses and tissue repair.
- Understanding their behavior after surgical stress is important for optimizing patient recovery.
Purpose of the Study:
- To investigate the effects of surgery on monocyte function and count.
- To assess changes in in vitro monocyte lysozyme production (IVMLP) and peripheral monocyte counts following surgical procedures.
Main Methods:
- Assessed IVMLP in 10 male patients preoperatively and postoperatively.
- Monitored IVMLP in 6 matched controls.
- Utilized a Technicon Hemalog D counter for accurate peripheral monocyte counts in patients and controls.
Main Results:
- Surgical patients exhibited a significant increase in IVMLP at 6-10 hours postoperatively, returning to baseline by 10 days.
- Peripheral monocyte counts were elevated in surgical patients at 36-48 hours and persisted for up to 10 days.
- Control groups showed stable IVMLP and monocyte counts throughout the study period.
Conclusions:
- Surgery induces both qualitative (IVMLP) and quantitative (monocyte counts) changes in mononuclear phagocytes.
- These alterations in monocyte behavior may influence the early stages of wound healing.