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Relationships of bilirubin binding parameters
Clinical Chemistry
|March 1, 1982
Summary
The peroxidase and hematofluorometer methods accurately measure bilirubin binding status in neonates. These methods show strong linear correlations with bilirubin-bound albumin and loosely bound bilirubin concentrations.
Area of Science:
- Clinical Chemistry
- Neonatal Medicine
- Biophysics
Background:
- Bilirubin binding status is crucial for assessing neonatal jaundice risk.
- Existing methods for measuring bilirubin binding capacity have limitations.
Purpose of the Study:
- To evaluate the validity of the peroxidase method for apparent unbound bilirubin (U) and the bilirubin hematofluorometer for albumin-bound bilirubin (B) and reserve binding capacity (R).
- To assess the correlation between these measurements and bilirubin binding status in neonatal blood specimens.
Main Methods:
- Measured U, T, B, and R in 164 neonatal specimens and artifactual specimens.
- Analyzed linear correlations between U and (B/R), and U and (T--B) (loosely bound bilirubin).
Main Results:
- Strong linear correlations (r=0.99, r=0.87) were found between U and (B/R) in both specimen types.
- Excellent correlation (r=0.96) observed between U and (T--B) in prepared specimens, supported by a low-affinity binding model.
- Significant correlation (r=0.72) between U and (T--B) in clinical specimens.
Conclusions:
- Both peroxidase and hematofluorometer methods provide valid measurements of bilirubin binding status.
- The findings support the mass action law and a simple model for low-affinity bilirubin binding in blood.