Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Intracochlear microprobe analysis

R C Bone, A F Ryan

    The Laryngoscope
    |April 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Energy dispersive x-ray analysis (EDXA) offers a precise method for measuring ion concentrations in the cochlea. This technique preserves sample integrity, preventing diffusion and enabling detailed analysis of auditory structures.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [The role of innate immunity in otitis media].

    HNO·2018
    Same author

    Hair cell stereociliary bundle regeneration by espin gene transduction after aminoglycoside damage and hair cell induction by Notch inhibition.

    Gene therapy·2016
    Same author

    BDNF gene therapy induces auditory nerve survival and fiber sprouting in deaf Pou4f3 mutant mice.

    Scientific reports·2012
    Same author

    Regulation of POU4F3 gene expression in hair cells by 5' DNA in mice.

    Neuroscience·2011
    Same author

    A specific arthritis with pericarditis affecting horses in Tasmania.

    Australian veterinary journal·2010
    Same author

    Histone deacetylase inhibition enhances adenoviral vector transduction in inner ear tissue.

    Neuroscience·2010
    Same journal

    Association Between the Modified Frailty Index and Short-Term Total Thyroidectomy Complications.

    The Laryngoscope·2026
    Same journal

    Discrimination of Pairs of Chemosensory Stimuli in Relation to Respiration.

    The Laryngoscope·2026
    Same journal

    What Safety Precautions Are Recommended When Lasering in the Airway?

    The Laryngoscope·2026
    Same journal

    Success of Anterior Ethmoidal Artery Flaps for Nasal Septal Perforation Repair: A Systematic Review.

    The Laryngoscope·2026
    Same journal

    Laryngeal IgG4-Related Disease: A Systematic Review of Clinical Features and Management.

    The Laryngoscope·2026
    Same journal

    Elevated BMI Is Not Associated With Adverse Outcomes in Open Airway Reconstruction.

    The Laryngoscope·2026
    See all related articles

    Area of Science:

    • Otoacoustic Emissions
    • Auditory Physiology
    • Biomedical Engineering

    Background:

    • Accurate measurement of ionic concentrations is crucial for understanding cochlear function.
    • Previous methods faced challenges with sample diffusion and limited spatial resolution.

    Purpose of the Study:

    • To introduce and evaluate Energy Dispersive X-ray Analysis (EDXA) as a method for assessing ionic concentrations in the cochlea.
    • To highlight the advantages of EDXA for analyzing small cochlear subregions and tissues.

    Main Methods:

    • Rapid freezing and lyophilization to obtain crystalline fluid samples from cochlear spaces.
    • Scanning electron microscopy for direct visualization and simultaneous biochemical analysis.
    • Energy Dispersive X-ray Analysis (EDXA) for determining relative ionic concentrations.

    Related Experiment Videos

    Main Results:

    • EDXA successfully analyzed ionic concentrations in perilymph, endolymph, stria vascularis, and the tectorial membrane.
    • The solid-state analysis prevented ion diffusion, ensuring sample integrity.
    • Simultaneous visualization and analysis confirmed the source of collected data.

    Conclusions:

    • EDXA is a valuable tool for cochlear physiologists, providing accurate ionic concentration data.
    • The method overcomes diffusion limitations, enabling precise analysis of cochlear microenvironments.
    • EDXA facilitates detailed investigation into the ionic basis of auditory function.