[Serotonin independently stimulates fibroblast adhesion and multiplication in culture]

The influence of serotonin on the adhesion and growth processes in primary cultures of murine and human embryo fibroblasts and L-cells was studied separately. At the moment of seeding, serotonin shortened the time of adhesion of glass surface of these from approximately 4 to 1 or 2 hours and stimulated incorporation of tritiated thymidine and cell multiplication when introduced in 2- or 6-hour cultures. Adhesion- and growth- stimulatory activities were blocked by serotonin antagonists - cyproheptadine and tipindole, demonstrating that the specific binding of bioamine with cell receptors is essential in both the cases.