Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble HLA antigens present in normal human serum

R J Billing, M Safani, P Peterson

    Tissue Antigens
    |August 1, 1977
    PubMed
    Summary

    Human leukocyte antigen (HLA) antigens were identified in serum using complement-dependent cytotoxicity tests. Serum HLA-A9 showed higher titers and unique molecular properties compared to other HLA antigens.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Between unity and disparity: current treatment protocols for common orofacial clefts in European expert centres.

    International journal of oral and maxillofacial surgery·2024
    Same author

    Evaluation and recommendations of the oral health, oral function, and orofacial aesthetics-related measures of the ICHOM Standard Set for Cleft Lip and Palate.

    International journal of oral and maxillofacial surgery·2024
    Same author

    External control cohorts for the single-arm LIBRETTO-001 trial of selpercatinib in RET+ non-small-cell lung cancer.

    ESMO open·2022
    Same author

    Ultra-high field magnetic resonance imaging parameter mapping in the posterior horn of ex vivo human menisci.

    Osteoarthritis and cartilage·2018
    Same author

    Persisting CAM deformity is associated with early cartilage degeneration after Slipped Capital Femoral Epiphysis: 11-year follow-up including dGEMRIC.

    Osteoarthritis and cartilage·2018
    Same author

    Pancreas volume and fat fraction in children with Type 1 diabetes.

    Diabetic medicine : a journal of the British Diabetic Association·2016

    Area of Science:

    • Immunogenetics
    • Human serum protein analysis
    • Cellular immunology

    Background:

    • Human leukocyte antigen (HLA) antigens are crucial for immune response.
    • Previous studies focused on cellular HLA expression, with limited understanding of their presence in serum.
    • Detecting serum HLA antigens could offer new insights into immune system function and disease.

    Purpose of the Study:

    • To detect and characterize human leukocyte antigen (HLA) antigens in normal human serum.
    • To compare the characteristics of serum HLA antigens with those found on lymphocytes.
    • To investigate the unique properties of specific HLA antigens, such as HLA-A9, in serum.

    Main Methods:

    • Utilized complement-dependent cytotoxicity tests with monospecific HLA antisera to detect serum HLA antigens.
    • Compared antigen profiles in serum with those on donor lymphocytes.
    • Employed techniques to determine the molecular weight and fraction of serum HLA antigens, including high-density lipoprotein analysis and treatment with detergent and papain.

    Main Results:

    • HLA antigens were successfully detected in normal human serum.
    • Serum-derived HLA antigens matched those found on the donor's lymphocytes.
    • HLA-A9 exhibited higher inhibitory titers (1:8 to 1:32) in serum compared to other A- and B-locus antigens (≤1:4).
    • Serum HLA-A9 antigens were associated with the high-density lipoprotein fraction, with molecular weights consistent with solubilized cellular HLA antigens after specific treatments.

    Conclusions:

    • Normal human serum contains detectable HLA antigens that mirror those on lymphocytes.
    • HLA-A9 demonstrates distinct behavior in serum regarding concentration and molecular characteristics.
    • The findings suggest serum HLA antigens share structural similarities with cellular counterparts, potentially offering a new avenue for immunological research.

    Related Experiment Videos