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Bovine coronavirus structural proteins

B King, D A Brian

    Journal of Virology
    |May 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Researchers analyzed bovine coronavirus (BCV) structural proteins using isotopic labeling. They identified five polypeptide species, including four external glycoproteins and one internal phosphorylated protein, revealing key viral components.

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    Area of Science:

    • Virology
    • Molecular Biology
    • Protein Chemistry

    Background:

    • Bovine coronavirus (BCV) is an important animal pathogen.
    • Understanding the structural proteins of BCV is crucial for developing antiviral strategies.
    • Previous characterization of BCV virion proteins was limited.

    Purpose of the Study:

    • To identify and characterize the structural proteins of the Mebus strain of bovine coronavirus.
    • To determine the glycosylation and phosphorylation status of BCV virion polypeptides.
    • To ascertain the location (external vs. internal) of identified viral proteins within the virion structure.

    Main Methods:

    • Bovine coronavirus (Mebus strain) was propagated in tissue culture.
    • Virions were purified and solubilized using urea and sodium dodecyl sulfate.

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  • Polypeptides were resolved using polyacrylamide gel electrophoresis (PAGE).
  • Isotopic labeling with amino acids, glucosamine, and orthophosphate was employed.
  • Susceptibility to proteolytic cleavage and enzymatic iodination was assessed on intact virions.

    • Main Results:

    • Five distinct polypeptide species were identified in purified BCV virions.
    • Four species were identified as glycoproteins with molecular weights of 140,000, 120,000, 100,000, and 26,000.
    • These glycoproteins are located on the exterior of the virion envelope, as indicated by their susceptibility to protease and iodination.
    • The 140,000 MW glycoprotein appears to be a dimer formed by disulfide linkages.
    • A fifth species, a 52,000 MW phosphorylated protein, was identified as an internal virion component due to its resistance to protease and iodination.

    Conclusions:

    • The structural analysis revealed a complex composition of bovine coronavirus virions.
    • BCV possesses multiple external glycoproteins and at least one internal phosphorylated protein.
    • These findings provide a foundation for further research into BCV structure-function relationships and pathogenesis.