[Synthetic medium for culturing Clostridium sporogenes]

Clostridium sporogenes was shown to be capable of good stable-growth and biomass accumulation up to 5 mg/ml (dry weight) in a chemically defined medium. Along with glucose, the culture required the following amino acids for its growth: arginine, phenylalanine, glycine tyrosine, leucine, isoleucine, valine, tryptophan, and methionine. The culture could grow in the absence of inorganic nitrogen but required such elements as phosphorus, iron and magnesium. Addition of cysteine to the medium accelerated the growth and increased the biomass yield. Leucine at a concentration over 0.1% inhibited the growth. Two vitamins, viz. biotin and p-aminobenzoic acid, are sufficient for the growth of the culture.