A GLC-nitrogen phosphorous detector assay for trifluoperazine in plasma

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Accurate quantification of trifluoperazine in plasma is essential for effective therapeutic drug monitoring and understanding its pharmacokinetics.
• Existing methods may have limitations in sensitivity, specificity, or accessibility.

Purpose of the Study:

• To develop and validate a sensitive and reliable gas-liquid chromatography-nitrogen-phosphorus detector (GLC-NPD) method for the quantitative determination of trifluoperazine in human plasma.
• To compare the performance of the developed GLC-NPD method with established techniques like GLC-mass spectrometry and radioimmunoassay.

Main Methods:

• Organic extraction of trifluoperazine and prochlorperazine (internal standard) from basified plasma.
• Evaporation of the organic solvent, reconstitution in methyl alcohol, and subsequent GLC-NPD analysis.
• Generation of standard curves for trifluoperazine over a concentration range of 0.5 to 15 ng/ml.

Main Results:

• The GLC-NPD method demonstrated linearity over the tested concentration range (0.5-15 ng/ml) with an overall coefficient of variation of 5.3%.
• The method achieved a determination limit of 0.5 ng/ml for trifluoperazine in plasma.
• The method was successfully applied to plasma samples from a healthy volunteer after oral administration of trifluoperazine.

Conclusions:

• The developed GLC-NPD method provides a sensitive, accurate, and reliable means for quantifying trifluoperazine in plasma.
• This method is suitable for pharmacokinetic studies and therapeutic drug monitoring of trifluoperazine.
• The GLC-NPD method offers a viable alternative to GLC-mass spectrometry and radioimmunoassay for trifluoperazine analysis.