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Dissociation between cutaneous basophil hypersensitivity and basophil sensitization
Journal of Immunology (Baltimore, Md. : 1950)
|September 1, 1982
Summary
Cutaneous basophil hypersensitivity (CBH) in guinea pigs showed a dissociation between in vivo skin tests and in vitro basophil sensitization early after immunization. This suggests T-cell mediation of early CBH, not antibodies.
Area of Science:
- Immunology
- Cellular immunology
Background:
- Cutaneous basophil hypersensitivity (CBH) is an immune response involving basophils.
- Understanding the kinetics and mediators of CBH is crucial for immune response studies.
Purpose of the Study:
- To investigate the antigen-specific sensitization of peripheral basophils in guinea pigs.
- To examine the relationship between in vivo CBH and in vitro basophil histamine release.
Main Methods:
- Guinea pigs were sensitized with keyhole limpet hemocyanin (KLH) or ovalbumin (OA).
- In vitro histamine release assay was performed on isolated basophils.
- In vivo 24-hr skin tests were conducted to assess CBH.
- Different immunization routes (intradermal vs. footpad with adjuvant) were compared.
Main Results:
- Basophils did not release histamine in vitro on day 7 but did on day 9 post-sensitization.
- In vivo CBH skin tests showed a typical response on both days 7 and 9.
- Immunization with adjuvant led to earlier or lower-dose basophil sensitization.
- A transient blood basophilia was observed, dependent on immunization route, antigen, and time.
Conclusions:
- A dissociation exists between in vivo CBH and in vitro basophil sensitization early post-immunization.
- Early CBH appears T-cell mediated, with antibodies playing a potential role later.
- The route of immunization significantly influences the timing and detection of basophil sensitization.