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[Cholesterol esterification in human plasma]

M Pocovi, J M Ordovas, F Grande

    Revista Espanola De Fisiologia
    |June 1, 1982
    PubMed
    Summary
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    This study measured the initial rates of lecithin: cholesterol acyltransferase (LCAT) activity in human plasma using gas-liquid chromatography. Factors like sex and cholesterol levels influence the rate of cholesterol esterification.

    Area of Science:

    • Biochemistry
    • Clinical Chemistry

    Context:

    • Lecithin: cholesterol acyltransferase (LCAT) plays a crucial role in reverse cholesterol transport.
    • Understanding LCAT activity is vital for assessing lipid metabolism and cardiovascular health.

    Purpose:

    • To measure the initial reaction rates of LCAT in human plasma.
    • To investigate the influence of sex, total cholesterol, HDL cholesterol, and unesterified cholesterol on LCAT activity.

    Summary:

    • Initial rates of LCAT-catalyzed reactions were determined in plasma from young adult males and females.
    • A gas-liquid chromatography method was employed, demonstrating linearity up to approximately 75 minutes.
    • The study identified significant influences of sex and various cholesterol concentrations on plasma cholesterol esterification rates.

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    Impact:

    • Provides a reliable method for quantifying LCAT initial rates in human plasma.
    • Highlights the impact of demographic and lipid profile factors on cholesterol esterification.
    • Contributes to a deeper understanding of lipid metabolism and potential cardiovascular risk factors.