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Updated: Aug 8, 2026

Cholesterol Efflux Assay
Published on: March 6, 2012
[Cholesterol esterification in human plasma]
Abstract:
The initial rates of the reaction catalized by the lecithin: cholesterol acyltransferase, were measured in the plasmas of two groups of normal subjects (20 males and 20 females) of the same age (average 20 years). The determination of the initial rate of lecithin: cholesterol acyltransferase reaction in human plasma was measured by a method based on the determination of plasma unesterified cholesterol by gas-liquid chromatography. With this technique the plasma esterification reaction is linear to approx. 75 min. The influence of sex, concentration of cholesterol total, high density lipoprotein cholesterol and unesterified cholesterol on the initial rate of plasma cholesterol esterification has been shown.
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