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[Crabtree effect caused by ketoses in isolated rat hepatocytes]
Revista Espanola De Fisiologia
|January 1, 1982
Summary
Fructose and tagatose inhibit cellular respiration in rat hepatocytes. Fructose also significantly increased aerobic glycolysis, indicated by lactate production, suggesting its unique metabolic impact.
Area of Science:
- Biochemistry
- Cellular Metabolism
- Hepatology
Background:
- Hepatocytes are crucial for metabolic regulation.
- Understanding substrate utilization is key to metabolic research.
- Investigating the effects of different sugars on liver cell function is important.
Purpose of the Study:
- To investigate the impact of various carbohydrates on oxygen consumption and glycolytic activity in isolated rat hepatocytes.
- To determine how fructose, tagatose, D-glyceraldehyde, glucose, L-sorbose, and glycerol affect cellular respiration and lactate production.
Main Methods:
- Isolation of hepatocytes from fed rats.
- Measurement of oxygen uptake using respirometry.
- Assessment of glycolytic activity via lactate production.
- Incubation of hepatocytes with different carbohydrate substrates.
Main Results:
- Fructose (38%) and tagatose (31%) significantly inhibited cellular respiration.
- D-glyceraldehyde induced a minor Crabtree effect.
- Glucose, L-sorbose, and glycerol did not alter oxygen consumption.
- Fructose incubation led to high aerobic glycolysis, evidenced by increased lactate production.
Conclusions:
- Fructose and tagatose exhibit inhibitory effects on hepatocyte respiration.
- Fructose uniquely promotes aerobic glycolysis in hepatocytes.
- Different hexoses and related compounds have distinct metabolic effects on liver cells.