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[A method for the gas chromatographic determination of long and medium chain free fatty acids in serum]
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|November 1, 1982
Abstract:
Free fatty acids in serum were determined by gas chromatography after esterification with boron tri-fluoride-methanol. Following prepurification of the serum lipid fractions by thin layer chromatography, the described analytical procedure selectively determines the medium and long chain free fatty acids. Accuracy and precision were tested. The method is suitable for the analysis of fatty acid kinetics in blood, following lipid infusions.